Journal: Scientific Reports
Article Title: Melanocyte Chitosan/Gelatin Composite Fabrication with Human Outer Root Sheath-Derived Cells to Produce Pigment
doi: 10.1038/s41598-019-41611-5
Figure Lengend Snippet: SP-gating strategy and cell cultivation. ( a ) Dissociated HuHF cells were separated into SP-p0 and different phases of the cell cycle (G0/G1, S, and G2/M) using SP technique. ( b ) Colony-forming,unit-like morphology in the primary culture of SP-p0. ( c ) SP-p4 cells displayed slender, multipolar cell protrusions with strong refractivity (4x). ( d ) SP-p4 cells displayed the typical melanocyte morphology (10x). SP: side population. G0-G2: cell cycle phases. Apo: apoptosis.
Article Snippet: Adherent cells were expanded and extended to the fourth passage (SP-p4) with melanocyte growth medium (M-GM, C-24010, Promocell, Heidelberg Germany) in an incubator at 37 °C under normoxic conditions (5% CO 2 , 95% air).
Techniques: